Simple Fools Guide
 

guide map

Navigate through the pipeline by clicking on any step in the flow chart
Guide Map

or DOWNLOAD THE FULL GUIDE [PDF]

 

Tissue   Sequencing   Computer   QC   Assembly   Annotation   Mapping   Expression   SNP

De Wit P, Pespeni MH, Ladner JTBarshis DJ, Seneca F, Jaris H, Overgaard Therkildsen N, Morikawa M and Palumbi SR (2012) The simple fool's guide to population genomics via RNA-Seq: an introduction to high-throughput sequencing data analysis.  Molecular Ecology Resources 12, 1058-1067.

Tissue Collection Computer Setup Quality Control De novo Assembly Mapping Reads BLAST SNP Detection Gene Expression Sequencing